Contract activities on ISS Russian Segment
The goal of this experiment is to determine whether natural killer (NK) cell activity is altered in microgravity.
NK cells are an important part of the immune system, since they are pre-programmed
to destroy virus infected cells and cancerous cells. Therefore it is important
for long term space exploration to characterize whether microgravity and spaceflight
conditions alter the function of NK cells, as this may affect the frequency at
which latent virus infections become reactivated or tumours develop in a long
duration space missions.
The experiment will measure the ability of NK cells from human peripheral blood
to lyse K562 target cells. This will be achieved by using 3H-Uridine labelled
formalin fixed K562 cells which will be mixed with NK cells in-flight, then incubated
for 24h. At the end of the incubation period cells will be filtered from the
medium in-flight, then transferred to refrigerated stowage until post flight
analysis. Post-flight, the amount of radiolabel in the intact target cells (on
the filter) and released from lysed cells (in the medium) will be measured, as
well as cytokine production by the target cells.
Determine whether natural killer (NK) cell activity is altered in microgravity.
- Quantify the activity of NK cells against K562 target cells under microgravity and 1g conditions;
- Quantify cytokine release from NK cells under microgravity and 1g conditions;
- Clearly distinguish the effect of microgravity from other spaceflight factors by the use of an on-board 1g centrifuge.
Science Hardware Used:
- KUBIK 1 incubator with the power cable;
- NKA Biocontainers (4);
- Cryogem-03M refrigerator/temperature controller container.
Biology containers (4) returned to the ground.
Extended data on the mechanism of latent virus infections and development of tumours in a long duration space missions.